Precise isolation
and analysis of circulating tumor cells (CTCs) from blood samples
offer considerable potential for cancer research and personalized
treatment. In what follows, a filter deterministic lateral
displacement (DLD) microchip is designed to achieve one-step and
label-free CTCs isolation with high throughput, which can enhance
performance for size-based cell separation, and achieved high
separation efficiency, high cell purity, high cell viability and
high processing rate. The CFD-Chip platform isolated CTCs with good
viability, enabling direct downstream analysis with single-cell RNA
sequencing. Transcriptome analysis of enriched CTCs identified new
subtypes of CTCs such as tumor cell-leukocyte fusion cells,
providing insights into cancer diagnostics and therapeutics.
